Difference between revisions of "Part:BBa J100065:Design"

 
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J100065 short</partinfo>
 
<partinfo>BBa_J100065 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Because the riboswitch must be directly beside the gene of interest (the start codon is used in the folding of the riboswitch), we added a BsaI recognition site.  This allows the riboswitch to be connected directly to a gene of interest using the Golden Gate Assembly method (if the first nucleotide after the start codon of the gene of interest is a C, we made it for use with superfolder GFP).
+
Designed to permit translation in the presence of theophylline.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
http://www.ncbi.nlm.nih.gov/pubmed?term=Synthetic%20Riboswitches%20That%20Induce%20Gene%20Expression%20in%20Diverse%20Bacterial%20Species
+
Synthetic part taken from reference below.
  
 
===References===
 
===References===
 +
 +
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988590/?tool=pubmed

Latest revision as of 13:52, 12 June 2012

Synthetic Riboswitch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 193
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 194
    Illegal PstI site found at 208
    Illegal NotI site found at 7
    Illegal NotI site found at 201
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 194
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 194
    Illegal PstI site found at 208
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 187


Design Notes

Designed to permit translation in the presence of theophylline.

Source

Synthetic part taken from reference below.

References

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988590/?tool=pubmed