Difference between revisions of "Part:BBa K896900:Design"
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===Design Notes===
 
===Design Notes===
 +
PCR primers (TM:55C):
 
  X174_pD_FP: gcttctag cattttgttcatggtagagattctct
 
  X174_pD_FP: gcttctag cattttgttcatggtagagattctct
 
  X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga
 
  X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga
−
 
−
 
  
 
===Source===
 
===Source===
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===References===
 
===References===
−
Sorensen S. E. ''et al'' (1998) Identification of the in vivo promoters of bacteriophages S13 and phi X174 and measurement of their relative activities. Biochem Cell Biol. 76(4):625-36.
+
Sorensen S. E. ''et al'' (1998) [http://www.ncbi.nlm.nih.gov/pubmed/10099783 Identification of the in vivo promoters of bacteriophages S13 and phi X174 and measurement of their relative activities]. Biochem Cell Biol. 76(4):625-36.

Latest revision as of 03:42, 27 April 2012

pD and rbsD from ΦX174


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR primers (TM:55C): 

X174_pD_FP: gcttctag cattttgttcatggtagagattctct
X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga

Source

Bacteriophage ΦX174 RF DNA

References

Sorensen S. E. et al (1998) [http://www.ncbi.nlm.nih.gov/pubmed/10099783 Identification of the in vivo promoters of bacteriophages S13 and phi X174 and measurement of their relative activities]. Biochem Cell Biol. 76(4):625-36.