Difference between revisions of "Part:BBa K896900:Design"
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<partinfo>BBa_K896900 short</partinfo> | <partinfo>BBa_K896900 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | PCR primers (TM:55C): | |
| − | + | X174_pD_FP: gcttctag cattttgttcatggtagagattctct | |
| − | + | X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga | |
===Source=== | ===Source=== | ||
| − | + | Bacteriophage ΦX174 RF DNA | |
===References=== | ===References=== | ||
| + | Sorensen S. E. ''et al'' (1998) [http://www.ncbi.nlm.nih.gov/pubmed/10099783 Identification of the in vivo promoters of bacteriophages S13 and phi X174 and measurement of their relative activities]. Biochem Cell Biol. 76(4):625-36. | ||
Latest revision as of 03:42, 27 April 2012
pD and rbsD from ΦX174
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR primers (TM:55C):
X174_pD_FP: gcttctag cattttgttcatggtagagattctct X174_pD_RP: gct ctgcagcggccgctactagta aaaagagcgtggattactatctga
Source
Bacteriophage ΦX174 RF DNA
References
Sorensen S. E. et al (1998) [http://www.ncbi.nlm.nih.gov/pubmed/10099783 Identification of the in vivo promoters of bacteriophages S13 and phi X174 and measurement of their relative activities]. Biochem Cell Biol. 76(4):625-36.