Difference between revisions of "Part:BBa K896937:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | Primers used in the PCR: | |
| − | + | colicin_FP: gcttctag atgagcggtggcgatg(TM:56C) | |
| − | + | colicin_RP: gct ctgcagcggccgctactagta ttacttaccccgatgaatatcaatatgt (TM:56C) | |
| + | Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone. | ||
===Source=== | ===Source=== | ||
| − | + | pIGMS5, a new plasmid from clinical Escherichia coli strain | |
| − | + | ||
===References=== | ===References=== | ||
| + | Smorawinska,M. and Plucienniczak,A. Submitted (24-AUG-2006) Bioengineering, Institute of Biotechnology and Antibiotics, Staroscinska 5, Warsaw 02-516, Poland | ||
Latest revision as of 17:47, 26 April 2012
colicin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers used in the PCR:
colicin_FP: gcttctag atgagcggtggcgatg(TM:56C) colicin_RP: gct ctgcagcggccgctactagta ttacttaccccgatgaatatcaatatgt (TM:56C)
Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.
Source
pIGMS5, a new plasmid from clinical Escherichia coli strain
References
Smorawinska,M. and Plucienniczak,A. Submitted (24-AUG-2006) Bioengineering, Institute of Biotechnology and Antibiotics, Staroscinska 5, Warsaw 02-516, Poland