Difference between revisions of "Part:BBa K896937"

 
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<partinfo>BBa_K896937 short</partinfo>
 
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<partinfo>BBa_K896937 parameters</partinfo>
 
<partinfo>BBa_K896937 parameters</partinfo>
 
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===Design Notes===
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Primers used in the PCR:
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colicin_FP: gcttctag atgagcggtggcgatg(TM:56C)
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colicin_RP: gct ctgcagcggccgctactagta ttacttaccccgatgaatatcaatatgt (TM:56C)
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Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.

Revision as of 17:36, 26 April 2012

colicin

...

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers used in the PCR: 

colicin_FP: gcttctag atgagcggtggcgatg(TM:56C)

colicin_RP: gct ctgcagcggccgctactagta ttacttaccccgatgaatatcaatatgt (TM:56C) Digested the PCR product with XbaI and PstI, and ligated the digestion product with a gel purified XbaI,PstI digested pSB1A2 plasmid backbone.