Difference between revisions of "Part:BBa J176005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor. | + | * Protein Data Bank ID 2H5Q |
+ | |||
+ | This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012). | ||
+ | |||
+ | |||
+ | REFERENCES | ||
+ | * Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol. | ||
Revision as of 20:59, 5 April 2012
mCh
Codon optimized mCherry red fluorescent protein
Usage and Biology
- Protein Data Bank ID 2H5Q
This "monomeric cherry" (mCherry) red fluorescent protein is codon optimized for expression in human/ mammalian cells. Only a single copy is needed for detectable signal. We have assembled two copies in tandem to create a "bulky" protein for use as an artificial transcriptional repressor (Haynes et al. 2012).
REFERENCES
- Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) A sensitive switch for visualizing natural gene silencing in single cells. ACS Syn Biol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]