Difference between revisions of "Part:BBa J176027"

 
 
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<partinfo>BBa_J176027 short</partinfo>
 
<partinfo>BBa_J176027 short</partinfo>
  
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constitute CMV promoter;PCR cloned from pcDNA3.1+
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Constitutive CMV promoter; PCR cloned from pcDNA3.1+
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:Haynes_Promoters_mCh.png|thumb|400px|'''Comparison of human promoters in U2OS cells''']]
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* "Strong" expression compared to Ubc and HPK
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* Constitutive (does not require induction)
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The cytomegalovirus promoter (CMV) promoter drives expression at a level greater than the ubiquitin C promoter (Ubc) and human phosphoglycerate kinase (HPK) in mammalian cells.
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<br><br>
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REFERENCES
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* Will be added soon
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Latest revision as of 20:37, 5 April 2012

CMV

Constitutive CMV promoter; PCR cloned from pcDNA3.1+


Usage and Biology

Comparison of human promoters in U2OS cells
  • "Strong" expression compared to Ubc and HPK
  • Constitutive (does not require induction)

The cytomegalovirus promoter (CMV) promoter drives expression at a level greater than the ubiquitin C promoter (Ubc) and human phosphoglycerate kinase (HPK) in mammalian cells.

REFERENCES

  • Will be added soon


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]