Difference between revisions of "Part:BBa J176028"

 
 
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<partinfo>BBa_J176028 short</partinfo>
 
<partinfo>BBa_J176028 short</partinfo>
  
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constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO
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Constitutive human phosphoglycerate kinase promoter
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:Haynes_Promoters_mCh.png|thumb|400px|'''Comparison of human promoters in U2OS cells''']]
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* "Medium" strength expression compared to CMV
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* Constitutive (does not require induction)
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The human phosphoglycerate kinase (HPK) promoter drives expression at a level intermediate to the ubiquitin C promoter (Ubc) and the popular cytomegalovirus promoter (CMV) in mammalian cells.
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<br><br>
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REFERENCES
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* Will be added soon
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Latest revision as of 20:22, 5 April 2012

HPK

Constitutive human phosphoglycerate kinase promoter


Usage and Biology

Comparison of human promoters in U2OS cells
  • "Medium" strength expression compared to CMV
  • Constitutive (does not require induction)

The human phosphoglycerate kinase (HPK) promoter drives expression at a level intermediate to the ubiquitin C promoter (Ubc) and the popular cytomegalovirus promoter (CMV) in mammalian cells.

REFERENCES

  • Will be added soon


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 116
  • 1000
    COMPATIBLE WITH RFC[1000]