Difference between revisions of "Part:BBa K649401:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The BioBrick-Prefix of this part is for "ATG-bigging type" while this's started from "AAG." | |
| − | + | ||
| + | However, you can use EcoRI/XbaI to construct and this will work correctly (binding site is from 17th bp). | ||
===Source=== | ===Source=== | ||
Latest revision as of 07:48, 17 February 2012
Arg box
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The BioBrick-Prefix of this part is for "ATG-bigging type" while this's started from "AAG."
However, you can use EcoRI/XbaI to construct and this will work correctly (binding site is from 17th bp).
Source
obtained from MG1655(E.coli) by PCR.
References
Stefan Schuster, "A general definition of metabolic pathways useful for systematic organization and analysis of complex metabolic networks" Nature Biotechnology , vol. 18, p326-32 (2000)