Difference between revisions of "Part:BBa J176124:Design"
(→Design Notes) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J176124 short</partinfo> | <partinfo>BBa_J176124 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | * Built by Andrew Younger (Pam Silver lab undergrad intern) | |
| − | + | * An EM7-Zeocin resistance-polyA fragment (cut with BsmBI/ BstBI) was used to replace the puromycin region of the pcDNA3.1+ deltaCMV vector (<partinfo>BBa_J176122</partinfo>) | |
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 21:12, 19 December 2011
pcDNA3.1+ zeo
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal SpeI site found at 249
Illegal PstI site found at 271
Illegal NotI site found at 292 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal BglII site found at 12
Illegal BamHI site found at 1438
Illegal XhoI site found at 299 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271
Illegal NgoMIV site found at 737
Illegal NgoMIV site found at 1887
Illegal NgoMIV site found at 1948
Illegal NgoMIV site found at 2213 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 3738
Illegal SapI site found at 2658
Design Notes
- Built by Andrew Younger (Pam Silver lab undergrad intern)
- An EM7-Zeocin resistance-polyA fragment (cut with BsmBI/ BstBI) was used to replace the puromycin region of the pcDNA3.1+ deltaCMV vector (BBa_J176122)
Source
TBA