Difference between revisions of "Part:BBa J176124"

Revision as of 04:29, 11 December 2011

pcDNA3.1+ zeo

pcDNA3.1+ zeo/ ΔpCMV, based on pcDNA3.1+; no promoter; ampicillin and zeocin resistance
Aliases: AY2, MV8

Usage and Biology

Select with ampicillin in E. coli
Select with zeomycin in mammalian cells

Mammalian DNA transfection vector. Great for temporary expression of your gene of interest, but transfection isn't 100% (for us, around 10%). The zeocin marker enables zeocin selection of mammalian cells that carry the plasmid. Long term selection leads to random insertion: a population of cells that have the plasmid (or multiple copies) inserted into a random spot in one or more chromosomes.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal SpeI site found at 249
Illegal PstI site found at 271
Illegal NotI site found at 292
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal BglII site found at 12
Illegal BamHI site found at 1438
Illegal XhoI site found at 299
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 266
Illegal XbaI site found at 305
Illegal SpeI site found at 249
Illegal PstI site found at 271
Illegal NgoMIV site found at 737
Illegal NgoMIV site found at 1887
Illegal NgoMIV site found at 1948
Illegal NgoMIV site found at 2213
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 3738
Illegal SapI site found at 2658

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J176124 short</partinfo>
-pcDNA3.1+ zeo/ &#916;pCMV, based on pcDNA3.1+; no promoter; ampicillin and neomycin resistance<br>
+pcDNA3.1+ zeo/ &#916;pCMV, based on pcDNA3.1+; no promoter; ampicillin and zeocin resistance<br>
 Aliases: AY2, MV8
@@ Line 13: / Line 13: @@
 * Select with zeomycin in mammalian cells
-Mammalian DNA transfection vector. Great for temporary expression of your gene of interest, but transfection isn't 100% (for us, around 10%). The zeomycin marker enables zeomycin selection of mammalian cells that carry the plasmid. Long term selection leads to random insertion: a population of cells that have the plasmid (or multiple copies) inserted into a random spot in one or more chromosomes.
+Mammalian DNA transfection vector. Great for temporary expression of your gene of interest, but transfection isn't 100% (for us, around 10%). The zeocin marker enables zeocin selection of mammalian cells that carry the plasmid. Long term selection leads to random insertion: a population of cells that have the plasmid (or multiple copies) inserted into a random spot in one or more chromosomes.
 <br>