Difference between revisions of "Part:BBa J176008"
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* Include within the ORF of a protein that you need transport into the nucleus (e.g., a synthetic transcription factor) | * Include within the ORF of a protein that you need transport into the nucleus (e.g., a synthetic transcription factor) | ||
| − | * | + | * Fluorophore-NLS fusions are handy for making glowing nuclei |
PKKKRKV motif from the Simian Virus 40 Large T-antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. in our hands, the localization appears to be very efficient (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges. | PKKKRKV motif from the Simian Virus 40 Large T-antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. in our hands, the localization appears to be very efficient (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges. | ||
Revision as of 02:52, 11 December 2011
NLS
SV40 nuclear localization sequence
Usage and Biology
- Include within the ORF of a protein that you need transport into the nucleus (e.g., a synthetic transcription factor)
- Fluorophore-NLS fusions are handy for making glowing nuclei
PKKKRKV motif from the Simian Virus 40 Large T-antigen. When added to a eukaryotic protein, that protein localizes to the nucleus. in our hands, the localization appears to be very efficient (data coming soon). This tag is very handy for live cell fluorescent labeling, since image processing of glowing nuclei with nice defined edges is much simpler than quantifying diffuse signal in cell bodies with irregular edges.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]