Difference between revisions of "Part:BBa J176134"

Latest revision as of 01:59, 11 December 2011

miRts CCND1

miR-34 target sequence, based on the CCND1 3' UTR
Alias: FC02

Usage and Biology

This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part appears to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J176134 short</partinfo>
-miR-34 target sequence, based on the BCL2 3' UTR<br>
+miR-34 target sequence, based on the CCND1 3' UTR<br>
-Alias: FC01
+Alias: FC02
 <br>
@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part does not appear to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells. Perhaps this target requires higher levels of miR-34 to function.
+This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part appears to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells.
 <br>