Difference between revisions of "Part:BBa J176134"
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===Usage and Biology===
 
===Usage and Biology===
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This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part does not appear to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells. Perhaps this target requires higher levels of miR-34 to function.
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Revision as of 01:58, 11 December 2011

miRts CCND1

miR-34 target sequence, based on the BCL2 3' UTR
Alias: FC01


Usage and Biology

This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part does not appear to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells. Perhaps this target requires higher levels of miR-34 to function.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]