Difference between revisions of "Part:BBa J176133"

 
 
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<partinfo>BBa_J176133 short</partinfo>
 
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Insert this sequence into the 3' UTR of your gene construct (i.e., after the ORF) to place it under control of the natural microRNA miR-34 in human cells.
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miR-34 target sequence, based on the BCL2 3' UTR<br>
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Alias: FC01
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===Usage and Biology===
 
===Usage and Biology===
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This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part does not appear to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells. Perhaps this target requires higher levels of miR-34 to function.
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Latest revision as of 01:57, 11 December 2011

miRts BCL2

miR-34 target sequence, based on the BCL2 3' UTR
Alias: FC01


Usage and Biology

This part is intended to bring a synthetic gene under the control of miR-34 in human cells. A gene carrying a target site for a functional miR will be repressed by RNA interference. The target site works by placing it downstream of the ORF you want to repress. In our hands, a gene with this part does not appear to be repressed in one cell line (U2OS) where miR-34 is produced at a minimal level in proliferating cells. Perhaps this target requires higher levels of miR-34 to function.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]