Difference between revisions of "Part:BBa M36304:Design"
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Latest revision as of 07:17, 10 December 2011
Constitutive promoter with FseI restriction sites
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal NgoMIV site found at 20
Illegal NgoMIV site found at 93
Illegal NgoMIV site found at 109 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The strong promoter was surrounded by four cut sites to increase the chance of FseI cutting the promoter on either side.