Difference between revisions of "Part:BBa J176022"

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Human codon-optimized Anemonia majano cyan fluorescent protein (AmCyan1) is a relatively bright version of CFP (blue). In our hands it works very well in human U2OS osteosarcoma cells.
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Human codon-optimized Anemonia majano cyan fluorescent protein (AmCyan1) is a version of CFP (blue). In our hands it works very well in human U2OS osteosarcoma cells.
  
  

Latest revision as of 03:12, 9 December 2011

AmCyan

Human codon-optimized AmCyan1 from pAmCyan1-C1


Usage and Biology

  • Open reading frame only
  • Requires promoter and translation start (e.g., Kozak) for expression
  • Excitation = 458 nm, emission = 489 nm


Human codon-optimized Anemonia majano cyan fluorescent protein (AmCyan1) is a version of CFP (blue). In our hands it works very well in human U2OS osteosarcoma cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]