Difference between revisions of "Part:BBa J176022"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J176022 short</partinfo> | <partinfo>BBa_J176022 short</partinfo> | ||
− | + | Human codon-optimized AmCyan1 from pAmCyan1-C1 | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | * Open reading frame only | ||
+ | * Requires promoter and translation start (e.g., Kozak) for expression | ||
+ | * Excitation = 458 nm, emission = 489 nm | ||
+ | <br> | ||
+ | |||
+ | Human codon-optimized Anemonia majano cyan fluorescent protein (AmCyan1) is a version of CFP (blue). In our hands it works very well in human U2OS osteosarcoma cells. | ||
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Latest revision as of 03:12, 9 December 2011
AmCyan
Human codon-optimized AmCyan1 from pAmCyan1-C1
Usage and Biology
- Open reading frame only
- Requires promoter and translation start (e.g., Kozak) for expression
- Excitation = 458 nm, emission = 489 nm
Human codon-optimized Anemonia majano cyan fluorescent protein (AmCyan1) is a version of CFP (blue). In our hands it works very well in human U2OS osteosarcoma cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]