Difference between revisions of "Part:BBa J176022:Design"
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* PCR-cloned from pAmCyan1-C1 (Clontech) | * PCR-cloned from pAmCyan1-C1 (Clontech) | ||
* Primers add a XbaI site upstream and SpeI/NotI/PstI downstream: | * Primers add a XbaI site upstream and SpeI/NotI/PstI downstream: | ||
| − | ** Forward 5- | + | ** Forward 5'-CCTTTCTAGAatggccctgtccaac |
| + | ** Reverse 5'-AAGGCTGCAGCGGCCGCTACTAGTgaagggcaccacgga | ||
| + | * Mutated at position 510 to remove a PstI site (site directed mutagenesis primer 5'-gatgctgcaAggcggcggca) | ||
| + | |||
===Source=== | ===Source=== | ||
| + | * pAmCyan1-C1 (Clontech) | ||
| − | |||
===References=== | ===References=== | ||
| + | * Matz, M. V., et al. (1999) Nature Biotech. 17:969–973 | ||
Latest revision as of 23:25, 8 December 2011
AmCyan
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- PCR-cloned from pAmCyan1-C1 (Clontech)
- Primers add a XbaI site upstream and SpeI/NotI/PstI downstream:
- Forward 5'-CCTTTCTAGAatggccctgtccaac
- Reverse 5'-AAGGCTGCAGCGGCCGCTACTAGTgaagggcaccacgga
- Mutated at position 510 to remove a PstI site (site directed mutagenesis primer 5'-gatgctgcaAggcggcggca)
Source
- pAmCyan1-C1 (Clontech)
References
- Matz, M. V., et al. (1999) Nature Biotech. 17:969–973