Difference between revisions of "Part:BBa J176022"

 
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<partinfo>BBa_J176022 short</partinfo>
 
<partinfo>BBa_J176022 short</partinfo>
  
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cyan fluorescent protein from PamCyan-1C1
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Human codon-optimized AmCyan1 from pAmCyan1-C1
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===Usage and Biology===
 
===Usage and Biology===
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* Open reading frame only
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* Requires promoter and translation start (e.g., Kozak) for expression
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* Excitation = 458 nm, emission = 489 nm
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<br>
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Human codon-optimized Anemonia majano cyan fluorescent protein (AmCyan1) is a relatively bright version of CFP (blue). In our hands it works very well in human U2OS osteosarcoma cells
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Revision as of 23:18, 8 December 2011

AmCyan

Human codon-optimized AmCyan1 from pAmCyan1-C1


Usage and Biology

  • Open reading frame only
  • Requires promoter and translation start (e.g., Kozak) for expression
  • Excitation = 458 nm, emission = 489 nm


Human codon-optimized Anemonia majano cyan fluorescent protein (AmCyan1) is a relatively bright version of CFP (blue). In our hands it works very well in human U2OS osteosarcoma cells


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]