Difference between revisions of "Part:BBa J176013:Design"

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In order of date published:
 
In order of date published:
  
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* (1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.
+
* (1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.<br>
−
<br>Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML; activity was compared to activation domains from other transcription factors
+
Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML; activity was compared to activation domains from other transcription factors
−
*
+
* (2005) Dreier B, Fuller RP, Segal DJ, Lund CV, Blancafort P, Huber A, Koksch B, Barbas CF 3rd. Development of Zinc Finger Domains for Recognition of the 5′-CNN-3′ Family DNA Sequences and Their Use in the Construction of Artificial Transcription Factors. J Biol Chem. 2005 Oct 21;280(42):35588-97.<br>
 +
Note: VP16 tetramer VP64 ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) fused to a zinc finger; synthetic construct added to NCBI database (GenBank: AAZ80416.1) in

Revision as of 02:08, 22 November 2011

VP64


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TBA


Source

TBA

References

In order of date published:

  • (1992) Seipel K, Georgiev O, Schaffner W. Different activation domains stimulate transcription from remote ('enhancer') and proximal ('promoter') positions. EMBO J. 1992 Dec;11(13):4961-8.

Note: Earliest reported test of a "modular" VP16 "core" activator, DALDDFDLDML; activity was compared to activation domains from other transcription factors

  • (2005) Dreier B, Fuller RP, Segal DJ, Lund CV, Blancafort P, Huber A, Koksch B, Barbas CF 3rd. Development of Zinc Finger Domains for Recognition of the 5′-CNN-3′ Family DNA Sequences and Their Use in the Construction of Artificial Transcription Factors. J Biol Chem. 2005 Oct 21;280(42):35588-97.

Note: VP16 tetramer VP64 ([DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]-GS-[DALDDFDLDML]) fused to a zinc finger; synthetic construct added to NCBI database (GenBank: AAZ80416.1) in