Difference between revisions of "Part:BBa K611023"
 
(5 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K611023 short</partinfo>
 
<partinfo>BBa_K611023 short</partinfo>
  
−
Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.  
+
Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs. These parts were characterized using K611013. The data below was collected in strain BW22826 which is, among other things, ΔAraBAD and ΔLacI. For more detailed information on this strain, it can be found here: http://cgsc.biology.yale.edu/Strain.php?ID=92290
 +
 
 +
<html>
 +
<img src="https://static.igem.org/mediawiki/parts/f/fb/UCD_Plot_allmuts_crop.png" width="600">
 +
<img src="https://static.igem.org/mediawiki/parts/e/ea/UCD_mut3_2D_crop.png" width="600">
 +
<img src="https://static.igem.org/mediawiki/parts/e/ee/UCD_R10WT.png" width="450">
 +
<img src="https://static.igem.org/mediawiki/parts/a/ac/UCD_R10mut3.png" width="450">
 +
</html><br>
 +
When induced with arabinose(as seen on the lower left axis), pBAD is derepressed allowing for transcription of the Lac repressor.  This is seen as a decrease in the reporter, GFP (on the left, vertical axis).  If IPTG is added(as seen on the right axis), it binds the Lac repressor which frees the promoter to transcribe.
  
 
https://static.igem.org/mediawiki/2011/0/0d/UCD_Partsreg_R10mut_seq.png
 
https://static.igem.org/mediawiki/2011/0/0d/UCD_Partsreg_R10mut_seq.png

Latest revision as of 05:23, 6 November 2011

LacI Promoter Variant #3

Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs. These parts were characterized using K611013. The data below was collected in strain BW22826 which is, among other things, ΔAraBAD and ΔLacI. For more detailed information on this strain, it can be found here: http://cgsc.biology.yale.edu/Strain.php?ID=92290


When induced with arabinose(as seen on the lower left axis), pBAD is derepressed allowing for transcription of the Lac repressor. This is seen as a decrease in the reporter, GFP (on the left, vertical axis). If IPTG is added(as seen on the right axis), it binds the Lac repressor which frees the promoter to transcribe.

UCD_Partsreg_R10mut_seq.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]