Difference between revisions of "Part:BBa K511905"

 
 
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<partinfo>BBa_K511905 short</partinfo>
 
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This MammoBlock composite device produces the rtTA3 repressor protein at a low, constitutive level that can be repressed by LacI variants.  
 
This MammoBlock composite device produces the rtTA3 repressor protein at a low, constitutive level that can be repressed by LacI variants.  
  
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https://static.igem.org/mediawiki/parts/8/86/Inverter_amplifier.jpg
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:17, 5 November 2011

Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device

This MammoBlock composite device produces the rtTA3 repressor protein at a low, constitutive level that can be repressed by LacI variants.

Inverter_amplifier.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal BglII site found at 615
    Illegal XhoI site found at 1014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]