Difference between revisions of "Part:BBa K511902"

Latest revision as of 15:35, 5 November 2011

rtTA3 Transactivator Generator (Hef1a-rtTA3) MammoBlock Device

This MammoBlock composite device produces the tetracycline-analogue-inducible rtTA3 transactivator at a constitutive level.

Graph showing the effect of doxycycline concentrations on the activation by constitutively expressed rtTA3 on the target TRE promoters heading two different reporters.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal BglII site found at 615
Illegal XhoI site found at 1014
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal NgoMIV site found at 749
Illegal AgeI site found at 127
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K511902 short</partinfo>
-This MammoBlock composite device produces the tetracycline-analogue-inducible rtTA3 transactivator at a low, constitutive level.
+This MammoBlock composite device produces the tetracycline-analogue-inducible rtTA3 transactivator at a constitutive level.
+Graph showing the effect of doxycycline concentrations on the activation by constitutively expressed rtTA3 on the target TRE promoters heading two different reporters.
+https://static.igem.org/mediawiki/parts/6/64/Hef1a_rtta_tre_mkate_and_tre_delta_graphs.jpg
 <!-- Add more about the biology of this part here