Difference between revisions of "Part:BBa K511808"

Latest revision as of 15:27, 5 November 2011

Repressible Yellow Fluorescent Protein Generator (Hef1a-LacO-EYFP) MammoBlock Device

This MammoBlock composite device produces the yellow fluorescent protein EYFP at a low, constitutive level driven by the Hef1a-LacO promoter and repressible by LacI variants.

Graph showing the repression of Hef1a-LacO:eYFP in the presence of LacI over a range of concentrations of IPTG.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal PstI site found at 1470
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal PstI site found at 1470
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal BglII site found at 615
Illegal XhoI site found at 1014
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal PstI site found at 1470
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 6
Illegal EcoRI site found at 1235
Illegal PstI site found at 361
Illegal PstI site found at 866
Illegal PstI site found at 1470
Illegal NgoMIV site found at 749
Illegal AgeI site found at 127
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K511808 short</partinfo>
 This MammoBlock composite device produces the yellow fluorescent protein EYFP at a low, constitutive level driven by the Hef1a-LacO promoter and repressible by LacI variants.
+Graph showing the repression of Hef1a-LacO:eYFP in the presence of LacI over a range of concentrations of IPTG.
+https://static.igem.org/mediawiki/parts/9/97/Hef1alacoeyfp_hef1alaci.jpg