Difference between revisions of "Part:BBa K515106"
 
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A composite part comprising of a J23100 promotor, holin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112805">BBa_K112805</a>) and endolysin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112806">BBa_K112806</a>).
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A composite part comprising of a J23100 promotor, holin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112805">BBa_K112805</a>) and endolysin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112806">BBa_K112806</a>). This part has been to designed to lyse any cell which does not contain part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515104"/>BBa_K515104</a> in the genome.
 
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<h2><b>Warning: This part is not RFC10 compatible</b></h2>
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<p>During Imperial College London's 2011 iGEM team's assembly of <a href="http://2011.igem.org/Team:Imperial_College_London/Project_Gene_Overview"/>Gene Guard</a> they encountered problems when cloning the holin-endolysin construct. They have found that there is an illegal XbaI site in the T4 Holin protein sequence originating from <a href="https://parts.igem.org/Part:BBa_K112808"><b>BBa_K112808</b></a>. This was also found in the <a href="https://parts.igem.org/cgi/sequencing/one_blast.cgi?id=10171"/>HQ's sequencing results</a> but the RFC10 compatibility tag has not been removed from the front page of this part.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:46, 4 November 2011

J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806

A composite part comprising of a J23100 promotor, holin (BBa_K112805) and endolysin (BBa_K112806). This part has been to designed to lyse any cell which does not contain part BBa_K515104 in the genome.

Warning: This part is not RFC10 compatible

During Imperial College London's 2011 iGEM team's assembly of Gene Guard they encountered problems when cloning the holin-endolysin construct. They have found that there is an illegal XbaI site in the T4 Holin protein sequence originating from BBa_K112808. This was also found in the HQ's sequencing results but the RFC10 compatibility tag has not been removed from the front page of this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
    Illegal AgeI site found at 1730
    Illegal AgeI site found at 1800
  • 1000
    COMPATIBLE WITH RFC[1000]