Difference between revisions of "Part:BBa K515106"
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A composite part comprising of a J23100 promotor, holin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112805">BBa_K112805</a>) and endolysin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112806">BBa_K112806</a>). This part has been to designed to lyse any cell which does not contain part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515104"/>BBa_K515104</a> in the genome.
 
A composite part comprising of a J23100 promotor, holin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112805">BBa_K112805</a>) and endolysin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K112806">BBa_K112806</a>). This part has been to designed to lyse any cell which does not contain part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515104"/>BBa_K515104</a> in the genome.
 
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<h2><b>Warning: This part is not RFC10 compatible</b></h2>
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<p>During Imperial College London's 2011 iGEM team's assembly of <a href="http://2011.igem.org/Team:Imperial_College_London/Project_Gene_Overview"/>Gene Guard</a> they encountered problems when cloning the holin-endolysin construct. They have found that there is an illegal XbaI site in the T4 Holin protein sequence. This was also found in the <a href="https://parts.igem.org/cgi/sequencing/one_blast.cgi?id=10171"/>HQ's sequencing results</a> but the RFC10 compatibility tag has not been removed from the front page of this part.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:44, 4 November 2011

J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806

A composite part comprising of a J23100 promotor, holin (BBa_K112805) and endolysin (BBa_K112806). This part has been to designed to lyse any cell which does not contain part BBa_K515104 in the genome.

Warning: This part is not RFC10 compatible

During Imperial College London's 2011 iGEM team's assembly of Gene Guard they encountered problems when cloning the holin-endolysin construct. They have found that there is an illegal XbaI site in the T4 Holin protein sequence. This was also found in the HQ's sequencing results but the RFC10 compatibility tag has not been removed from the front page of this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
    Illegal AgeI site found at 1730
    Illegal AgeI site found at 1800
  • 1000
    COMPATIBLE WITH RFC[1000]