Difference between revisions of "Part:pSB4A5:Experience"

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'''***Warning:***'''[[Part:pSB4A5|pSB4A5]] does not appear to be correct in any distribution wells.  The [http://2011.igem.org/Team:Washington 2011 Washington iGEM team] sequenced pSB4A5 from distributions in the past several years and found it to be in fact pSB1A2 - a high-copy, not a low-copy, plasmid.  The UW iGEM team constructed pGA4A5, similar to pSB4A5 but for Gibson-friendly cloning.  For more information, visit [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Tookit] section of the Washington 2011 iGEM team's wiki.
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'''***Warning:***'''[[Part:pSB4A5|pSB4A5]] does not appear to be correct in many distribution wells.  The [http://2011.igem.org/Team:Washington 2011 Washington iGEM team] sequenced pSB4A5 from distributions in the past several years and found it to be in fact pSB1A2 - a high-copy, not a low-copy, plasmid.  The UW iGEM team constructed pGA4A5, similar to pSB4A5 but for Gibson-friendly cloning.  For more information, visit [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Tookit] section of the Washington 2011 iGEM team's wiki.
 
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Revision as of 03:29, 3 November 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB4A5

User Reviews

UNIQa49bc2e93c827004-partinfo-00000000-QINU

•••••

Reshma Shetty

pSB4A5 has not been tested but is expected to be fully functional as a BioBrick standard vector. When miniprepped pSB4A5-I52002 gives high miniprep yield similar to a high copy plasmids.

•

User:Swanson

***Warning:***pSB4A5 does not appear to be correct in many distribution wells. The [http://2011.igem.org/Team:Washington 2011 Washington iGEM team] sequenced pSB4A5 from distributions in the past several years and found it to be in fact pSB1A2 - a high-copy, not a low-copy, plasmid. The UW iGEM team constructed pGA4A5, similar to pSB4A5 but for Gibson-friendly cloning. For more information, visit [http://2011.igem.org/Team:Washington/Magnetosomes/GibsonVectors Gibson Tookit] section of the Washington 2011 iGEM team's wiki.

UNIQa49bc2e93c827004-partinfo-00000003-QINU