Difference between revisions of "Part:BBa K649401"
Takuya 1613 (Talk | contribs) |
|||
| (5 intermediate revisions by 2 users not shown) | |||
| Line 4: | Line 4: | ||
This part contains the Arg Box. The Arg Box is the arginine operator capable of binding the arginine repressor. | This part contains the Arg Box. The Arg Box is the arginine operator capable of binding the arginine repressor. | ||
| − | [[Image: | + | [[Image:Arg_box_on_1C3.png|thumb|center|500px|Urea concentration detected in bacterial samples on pSB3K3<br /> |
| − | This work | + | This work was done by Natsuki Kubo.]]<br /> |
| + | As a way to derepress arginine biosynthesis we introduced Arg box on our part BBa_K649401 in addition to <i>rocF</i> gene on our part [https://parts.igem.org/Part:BBa_K649301 BBa_K649301]. In this case, more urea was produced compared that when only <i>rocF</i> gene was introduced. These results show that Arg boxes are effectively derepressing arginine production by deactivating the arginine repressor. | ||
| + | |||
| + | For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/data#1. our work in Tokyo_Tech 2011 wiki.] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 05:17, 29 October 2011
Arg box
This part contains the Arg Box. The Arg Box is the arginine operator capable of binding the arginine repressor.
As a way to derepress arginine biosynthesis we introduced Arg box on our part BBa_K649401 in addition to rocF gene on our part BBa_K649301. In this case, more urea was produced compared that when only rocF gene was introduced. These results show that Arg boxes are effectively derepressing arginine production by deactivating the arginine repressor.
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/data#1. our work in Tokyo_Tech 2011 wiki.]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 3
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]