Difference between revisions of "Part:BBa K572009"

 
 
(4 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K572009 short</partinfo>
 
<partinfo>BBa_K572009 short</partinfo>
  
..
+
It is an oxidoreductase-enzyme which catalyzes the conversion of beta-carotene to retinal in the presence of oxygen, according to the reaction:
 +
<br>beta-carotene + O2 <=> 2 retinal.<br/>
 +
The product of this enzymatic reaction, i.e Retinal is a VitaminA aldehyde. It is a polyene chromophore which binds to proteins called 'Opsins' (say Proteorhodopsin). This retinal-opsin complex allows microorganisms to convert light energy to metabolic energy.
 +
Hence in micro-organisms functionally expressing Proteorhodopsin, beta-carotene dioxygenase converts beta-carotene to Trans-retinal, which is required for the functioning of Proteorhodopsin.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
  
 
<!-- -->
 
<!-- -->
Line 17: Line 20:
 
<partinfo>BBa_K572009 parameters</partinfo>
 
<partinfo>BBa_K572009 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<hr/>
 +
<html>
 +
<img src=" https://static.igem.org/mediawiki/parts/a/a0/K572009_Page_1.jpg" width="750px" height="971px" />
 +
<img src="https://static.igem.org/mediawiki/parts/e/ee/K572009_Page_2.jpg" width="750px" height="971px" />
 +
</html>

Latest revision as of 23:31, 28 October 2011

Mouse Beta Carotene Dioxygenase

It is an oxidoreductase-enzyme which catalyzes the conversion of beta-carotene to retinal in the presence of oxygen, according to the reaction:
beta-carotene + O2 <=> 2 retinal.
The product of this enzymatic reaction, i.e Retinal is a VitaminA aldehyde. It is a polyene chromophore which binds to proteins called 'Opsins' (say Proteorhodopsin). This retinal-opsin complex allows microorganisms to convert light energy to metabolic energy. Hence in micro-organisms functionally expressing Proteorhodopsin, beta-carotene dioxygenase converts beta-carotene to Trans-retinal, which is required for the functioning of Proteorhodopsin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 82
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 82
    Illegal PstI site found at 220
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1443
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 82
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 82
    Illegal PstI site found at 220
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 418
    Illegal SapI site found at 999