Difference between revisions of "Part:BBa K537011"
 
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This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS 2007) which is fused to CheZ and Venus fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The theophylline riboswitch1-CheZ fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
 
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS 2007) which is fused to CheZ and Venus fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The theophylline riboswitch1-CheZ fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
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Note: All scar sites have been excluded in the annotations.
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There is a scar site between the Promoter:CheZ-Venus fusion and CheZ-Venus fusion:Double terminator. The scar sequence is as follows: ACTAGA
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A Freiburg fusion scar site exists between the CheZ and Venus sequence. The sequence is as follows: ACCGGC
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K537011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K537011 SequenceAndFeatures</partinfo>
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<html>
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<p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/parts/2/27/Datasheet1.pdf">here</a></big></p>
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<center><img src="https://static.igem.org/mediawiki/igem.org/5/5b/Datasheet1.jpg" width="910" vspace="15"></center>
  
  
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</html>
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K537011 parameters</partinfo>
 
<partinfo>BBa_K537011 parameters</partinfo>
 
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Latest revision as of 19:37, 28 October 2011

Promoter-Theophylline riboswitch 1-CheZ-Venus-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS 2007) which is fused to CheZ and Venus fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The theophylline riboswitch1-CheZ fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).

Note: All scar sites have been excluded in the annotations. There is a scar site between the Promoter:CheZ-Venus fusion and CheZ-Venus fusion:Double terminator. The scar sequence is as follows: ACTAGA

A Freiburg fusion scar site exists between the CheZ and Venus sequence. The sequence is as follows: ACCGGC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



For the pdf version of this datasheet, click here