Difference between revisions of "Part:BBa K537009"
 
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<partinfo>BBa_K537009 short</partinfo>
 
<partinfo>BBa_K537009 short</partinfo>
  
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This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS 2007) which is fused to CheZ and Venus fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The theophylline riboswitch1-CheZ fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
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This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS, 2007) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch1-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
  
 
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<partinfo>BBa_K537009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K537009 SequenceAndFeatures</partinfo>
  
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<p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/parts/2/27/Datasheet1.pdf">here</a></big></p>
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<center><img src="https://static.igem.org/mediawiki/parts/5/5b/Datasheet1.jpg" width="910" vspace="15"></center>
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K537009 parameters</partinfo>
 
<partinfo>BBa_K537009 parameters</partinfo>
 
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Latest revision as of 19:35, 28 October 2011

Promoter-Theophylline riboswitch 1-Venus-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type1) (Topp and Gallivan JACS, 2007) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch1-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



For the pdf version of this datasheet, click here