Difference between revisions of "Part:BBa K533012:Design"

(→‎Design Notes)
(→‎Design Notes)
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This plasmid is designed to increase the bio-safety level of biobricks. It contains a constitutive ccdB expression cassette and therefore is toxic to common ''E. coli'' strains. In this way, the spread of this plasmid is restricted.
 
This plasmid is designed to increase the bio-safety level of biobricks. It contains a constitutive ccdB expression cassette and therefore is toxic to common ''E. coli'' strains. In this way, the spread of this plasmid is restricted.
  
−
The vector also contains a mutated I-SceI site, which allows for elimation of mutation using our restriction-mutation system.
+
The vector also contains a mutated I-SceI site, which allows for elimation of mutation using our restriction-mutation system.[http://2011.igem.org/Team:Tsinghua/safety#Proposal_on_increased_safety|Safety proposal]
  
 
Do not use commonly used commercial competent cells when using this plasmid. ccdB-resistance strains are required.
 
Do not use commonly used commercial competent cells when using this plasmid. ccdB-resistance strains are required.

Revision as of 18:51, 28 October 2011

safety version of pSB1C3 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal BamHI site found at 2566
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2146


Design Notes

This plasmid is designed to increase the bio-safety level of biobricks. It contains a constitutive ccdB expression cassette and therefore is toxic to common E. coli strains. In this way, the spread of this plasmid is restricted.

The vector also contains a mutated I-SceI site, which allows for elimation of mutation using our restriction-mutation system.[http://2011.igem.org/Team:Tsinghua/safety#Proposal_on_increased_safety|Safety proposal]

Do not use commonly used commercial competent cells when using this plasmid. ccdB-resistance strains are required.

Source

derived from pSB1C3

References