Difference between revisions of "Part:BBa K533012:Design"

 
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<partinfo>BBa_K533012 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Do not use commonly used commercial competent cells when using this plasmid. Special strains are required.
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This plasmid is designed to increase the bio-safety level of biobricks. It contains a constitutive ccdB expression cassette and therefore is toxic to common ''E. coli'' strains. In this way, the spread of this plasmid is restricted.
  
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The vector also contains a mutated I-SceI site, which allows for elimation of mutation using our restriction-mutation system.
  
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Do not use commonly used commercial competent cells when using this plasmid. ccdB-resistance strains are required.
  
 
===Source===
 
===Source===

Revision as of 18:50, 28 October 2011

safety version of pSB1C3 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal BamHI site found at 2566
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2590
    Illegal XbaI site found at 2596
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2146


Design Notes

This plasmid is designed to increase the bio-safety level of biobricks. It contains a constitutive ccdB expression cassette and therefore is toxic to common E. coli strains. In this way, the spread of this plasmid is restricted.

The vector also contains a mutated I-SceI site, which allows for elimation of mutation using our restriction-mutation system.

Do not use commonly used commercial competent cells when using this plasmid. ccdB-resistance strains are required.

Source

derived from pSB1C3

References