Difference between revisions of "Part:BBa K627017"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The Protease was isolated from the human rhinovirus strain 14 and belongs to the class of 3C proteases. The PreSciccion protease which is selled by GE, Sigma and many other, and is used for tag cleavage is based on this protease. Its optimized to an maximum activity at 4°C. For kinetic data and other information please check the offers from GE and Sigma and see thier refernces. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 18:48, 28 October 2011
HRV 14_3C protease
Protease of the Human rhinovirus strain 14 protease class 3C. The iGEM restriction sites were removed via side directed mutation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 278
Illegal BglII site found at 466 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]