Difference between revisions of "Part:BBa K627017:Design"

(New page: __NOTOC__ <partinfo>BBa_K627012 short</partinfo> <partinfo>BBa_K627012 SequenceAndFeatures</partinfo> ===Design Notes=== This biobrick was built by PCR using the following PCR primers:<...)
 
 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K627012 short</partinfo>
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<partinfo>BBa_K627017 short</partinfo>
  
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<partinfo>BBa_K627012 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K627017 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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This biobrick was built by PCR using the following PCR primers:<br>
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The protease is flanked by the iGEM suffix and prefix based on the RFC25.
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* p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
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* o_Bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC
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===Source===
 
===Source===
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TorA signal sequence and the lactamase was amplified via PCR from pJC354, which originates from two iGEM compatible BioBricks: BBa_K208005 and BBa_I757010. The cleavage site was created, based on the infoarmations of the Brenda enzymes database, via 2 oligonucleotides ordered form SigmaAldtrich.
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The plasmid was received by the research group of Gunter Stier.
  
 
===References===
 
===References===
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ssTorA - https://parts.igem.org/Part:BBa_K208005<br>
 
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blaFL - https://parts.igem.org/Part:BBa_I757010<br>
 

Latest revision as of 18:43, 28 October 2011


HRV 14_3C protease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
    Illegal BglII site found at 466
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protease is flanked by the iGEM suffix and prefix based on the RFC25.


Source

The plasmid was received by the research group of Gunter Stier.

References