Difference between revisions of "Part:BBa K523024"

Latest revision as of 18:36, 28 October 2011

cex + cenA fusion (cxnA)

Cex exoglucanase from Cellulomonas fimi fused to CenA endoglucanase from the same species. The part contains, in order:

Edinburgh 2011 carried out some experiments on this part under the control of the lac promoter - see details at part BBa_K523025.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 524
Illegal NotI site found at 2222
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2135
Illegal XhoI site found at 1633
Illegal XhoI site found at 1882
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 157
Illegal NgoMIV site found at 530
Illegal NgoMIV site found at 1032
Illegal NgoMIV site found at 1413
Illegal NgoMIV site found at 2338
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 577
Illegal SapI.rc site found at 660

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K523024 short</partinfo>
-Cex exoglucanase from ''Cellulomonas fimi'' fused to CenA endoglucanase from the same species.
+Cex exoglucanase from ''Cellulomonas fimi'' fused to CenA endoglucanase from the same species. The part contains, in order:
+* The GH10 exoglucanase domain
+* A (possibly inactivated) cellulose binding domain
+* The GH6 endoglucanase domain
+<font color="red">Edinburgh 2011 carried out some experiments on this part under the control of the lac promoter - see details at part <partinfo>BBa_K523025</partinfo>.</font>
 <!-- Add more about the biology of this part here
@@ Line 9: / Line 15: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'>'''Sequence and Features'''</span>
 <partinfo>BBa_K523024 SequenceAndFeatures</partinfo>