Difference between revisions of "Part:BBa K523025"
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Revision as of 18:33, 28 October 2011
Plac + RBS + cxnA (cex + cenA fusion)
Lac promoter and RBS, followed by Cex exoglucanase from Cellulomonas fimi fused to CenA endoglucanase from the same species. The coding sequence contains, in order:
- The GH10 exoglucanase domain
- A (possibly inactivated) cellulose binding domain
- The GH6 endoglucanase domain
In the process of creating this part, we tested putative correct clones on carboxymethylcellulose (CMC) plates, with Congo red dye. This test produces a zone of clearing if CMC is degraded by endoglucanase. We had three successful colonies:
Evidently we had three correct colonies (these had already been tested for their ability to degrade MUC, in an assay similar to that for BBa_K523016.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1148
Illegal NotI site found at 2846 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2759
Illegal XhoI site found at 2257
Illegal XhoI site found at 2506 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 781
Illegal NgoMIV site found at 1154
Illegal NgoMIV site found at 1656
Illegal NgoMIV site found at 2037
Illegal NgoMIV site found at 2962 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1201
Illegal SapI.rc site found at 1284