Difference between revisions of "Part:BBa K649105"

Latest revision as of 14:47, 28 October 2011

PlsrA-gfp-PlsrR-lsrR

Fluorescence intensity is decreased by LsrR repression.
This work is done by Hiroki Yoshise.

As we described in BBa_K649101, in this part, lsrK has mutation and does not work properly and oher genes work correctly.

We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a lsrR gene downstream of lsrA promoter-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed lsrA promoter. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. our work in Tokyo_Tech 2011 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2579
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2253
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 770

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K649105 short</partinfo>
-Median fluorescence intensity(MFI) of BBa_K649104(PlsrA-RBS-gfp) is 3-fold higher than that of BBa_K649105(PlsrA-gfp-PlsrR-lsrR).
+[[Image:lsrRBA.png|thumb|center|500px|Fluorescence intensity is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]
-[[Image:OD lsrR repression.png|thumb|center|400px|After four hours from OD590 reaching 0.15, we measured OD.<br>This work is done by Hiroki Yoshise.]]
-[[Image:LsrR repression.png|thumb|center|300px|Median fluorescence intensity(MFI) is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]
+As we described in [https://parts.igem.org/Part:BBa_K649101 BBa_K649101], in this part, <i>lsrK</i> has mutation and does not work properly and oher genes work correctly.
+We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a <i>lsrR</i> gene downstream of lsrA promoter-<i>gfp</i>.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed lsrA promoter. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.
-We confirmed that LsrR represses lsrA promoter.
-For this purpose, we constructed BBa_K649105(PlsrA-gfp-PlsrR-lsrR) and compared fluorescence intensity levels of BBa_K649104(PlsrA-RBS-gfp) and BBa_K649105.
+For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. our work in Tokyo_Tech 2011 wiki].
-For more information, see our work in Tokyo_Tech 2011 wiki