Difference between revisions of "Part:BBa K649104"

Latest revision as of 14:36, 28 October 2011

PlsrA-RBS-gfp

We characterized BBa_K649104 and BBa_K117002 in E.coli lsrR(-).

Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-gfp(negative control).
Strain used in this assay lacks lsrR.
This work is done by Takuya Tsubaki.

We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a gfp gene downstream of the promoter. Its fluorescence intensity was much higher than that of promoterless-gfp(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of lsrA promoter-gfp((BBa_K117002)-gfp) was almost the same as a promoterless-gfp(negative control), showing that lsrA promoter(BBa_K117002) does not work properly.

We improved previous lsrA promoter(BBa_K117002).our assay of BBa_K117002

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. our work in Tokyo_Tech 2011 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 770

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K649104 short</partinfo>
-[[Image:PlsrA_activity.png|thumb|center|700px|Median fluorescence intensity(MFI) of BBa_K649104 was much higher than that of promoterless-gfp(negative control).<br>This work is done by Takuya Tsubaki.]]
+We characterized BBa_K649104 and BBa_K117002 in <i>E.coli</i> <i>lsrR</i>(-).
-We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a gfp gene downstream of the promoter.  Its fluorescence intensity was much higher than that of promoterless-gfp(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of PlsrA(BBa_117002)-gfp was almost the same as a promoterless-gfp(negative control), showing that BBa_K117002 does not work properly.
+[[Image:PlsrA2.png|thumb|center|600px|Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-<i>gfp</i>(negative control).<br>Strain used in this assay lacks <i>lsrR</i>.<br>This work is done by Takuya Tsubaki.]]
+We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a <i>gfp</i> gene downstream of the promoter.  Its fluorescence intensity was much higher than that of promoterless-<i>gfp</i>(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of lsrA promoter-<i>gfp</i>((BBa_K117002)-<i>gfp</i>) was almost the same as a promoterless-<i>gfp</i>(negative control), showing that lsrA promoter(BBa_K117002) does not work properly.
@@ Line 10: / Line 13: @@
-For more information, see our work in Tokyo_Tech 2011 wiki
+For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. our work in Tokyo_Tech 2011 wiki].