Difference between revisions of "Part:BBa K649104"
 
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<partinfo>BBa_K649104 short</partinfo>
 
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We measured the transcriptional activity of our lsrA promoter by introducing a gfp gene downstream of the promoter.  Its fluorescence intensity was much higher than that from a promoter-less GFP negative control plasmid, showing that our new lsrA promoter works.  
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We characterized BBa_K649104 and BBa_K117002 in <i>E.coli</i> <i>lsrR</i>(-).
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[[Image:PlsrA2.png|thumb|center|600px|Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-<i>gfp</i>(negative control).<br>Strain used in this assay lacks <i>lsrR</i>.<br>This work is done by Takuya Tsubaki.]]
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We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a <i>gfp</i> gene downstream of the promoter.  Its fluorescence intensity was much higher than that of promoterless-<i>gfp</i>(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of lsrA promoter-<i>gfp</i>((BBa_K117002)-<i>gfp</i>) was almost the same as a promoterless-<i>gfp</i>(negative control), showing that lsrA promoter(BBa_K117002) does not work properly.
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We improved previous lsrA promoter(BBa_K117002).[https://parts.igem.org/Part:BBa_K117002:Experience our assay of BBa_K117002]
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For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. our work in Tokyo_Tech 2011 wiki].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:36, 28 October 2011

PlsrA-RBS-gfp

We characterized BBa_K649104 and BBa_K117002 in E.coli lsrR(-).

Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-gfp(negative control).
Strain used in this assay lacks lsrR.
This work is done by Takuya Tsubaki.


We measured the transcriptional activity of our lsrA promoter(BBa_K649100) by introducing a gfp gene downstream of the promoter. Its fluorescence intensity was much higher than that of promoterless-gfp(negative control), showing that our new lsrA promoter works. Moreover,fluorescence intensity of lsrA promoter-gfp((BBa_K117002)-gfp) was almost the same as a promoterless-gfp(negative control), showing that lsrA promoter(BBa_K117002) does not work properly.


We improved previous lsrA promoter(BBa_K117002).our assay of BBa_K117002


For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. our work in Tokyo_Tech 2011 wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 770