Difference between revisions of "Part:BBa K649100"

Latest revision as of 14:28, 28 October 2011

LsrA promoter

BBa_K649100 contains lsrA promoter and RBS.

lsrA promoter is repressed by LsrR. In the presense of Phospho-AI2 the transcription of downstream gene is activated.

To characterize this promoter, we constructed the reporter part BBa_K649104.

Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-gfp(negative control).
Strain used in this assay lacks lsrR.
This work is done by Takuya Tsubaki.

If you want to see detailed infomation, please click here.

We inproved previous lsrA promoter(BBa_K117002)).our assay of BBa_K117002

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. our work in Tokyo_Tech 2011 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 BBa_K649100 contains lsrA promoter and RBS.<br>
 lsrA promoter is repressed by LsrR. In the presense of Phospho-AI2 the transcription of downstream gene is activated.
-To characterize this promoter, we constructed the reporter part BBa_K649104. If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649104 here.]
+To characterize this promoter, we constructed the reporter part BBa_K649104. <br>
+[[Image:PlsrA2.png|thumb|center|600px|Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-<i>gfp</i>(negative control).<br>Strain used in this assay lacks <i>lsrR</i>.<br>This work is done by Takuya Tsubaki.]]<br>
+If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649104 here.]