Difference between revisions of "Part:BBa K537010"
 
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This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
 
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
  
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<center><img src="https://static.igem.org/mediawiki/2011/4/47/Datasheet2a.jpg" width="910" vspace="15"></center>
 
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<p>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/parts/f/f5/Datasheet2.pdf">here</a></p>
 
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<p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/igem.org/f/f5/Datasheet2.pdf">here</a></big></p>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K537010 parameters</partinfo>
 
<partinfo>BBa_K537010 parameters</partinfo>
 
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Latest revision as of 12:44, 28 October 2011

Promoter-Theophylline riboswitch 2-Venus-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




For the pdf version of this datasheet, click here