Difference between revisions of "Part:BBa K649400"

Line 6: Line 6:
 
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm our work in Tokyo_Tech 2011 wiki].  
 
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm our work in Tokyo_Tech 2011 wiki].  
  
 
+
[[Image:ispS_assay_result.png|thumb|left|400px|isoprene synthesized by ''E. coli'' introduced ''ispS'' gene.<br>This work is done by Yuto Sugiuchi.]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:38, 28 October 2011

isoprene synthase (ispS)

This part contains the gene isoprene synthase(ispS) and RBS. The ispS catalyzes the conversion of dimethylallyl diphoshate to isoprene.
To characterize this part, we constructed the composite part, BBa_K649303. BBa_K649303 is composed of PlacIQ(BBa_I14032) and RBS-ispS(BBa_K649400).
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm our work in Tokyo_Tech 2011 wiki].

isoprene synthesized by E. coli introduced ispS gene.
This work is done by Yuto Sugiuchi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 762
    Illegal BglII site found at 1317
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]