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http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/GC-Assay#AP
 
 
===User Reviews===
 
===User Reviews===
 
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Revision as of 04:54, 28 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K649303

isoprene synthesized by E. coli introduced ispS gene.
This work is done by Yuto Sugiuchi.

Each bacterial sample was grown in a 500 mL flask containing 100 mL LB media. Cultures were grown at 37℃ and then induced by 0.5 mM IPTG when OD600 reached 0.6. After 4 hours of induction, 50 mL of headspace gas was taken by absorbing material (mini-PAT including Tenax: Japan Analytical Industry Co., Ltd) and injected into GC-MS.

We calculated the amount of isoprene by calibration date we obtained. [http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/GC-Assay#AP calibration date].


User Reviews

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