Difference between revisions of "Part:BBa K649301"

Revision as of 04:07, 27 October 2011

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.

Urea concentration in growth media 1 hour after IPTG induction.
This work is done by Natsuki Kubo.

Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +).
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 230
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 This work is done by Natsuki Kubo.]]
-Introduction of BBa_K649301 containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +). This part produced urea more efficiently in strain JD24293 (<span style="font-style:italic;">argR</span> -).<br /><br />
+Introduction of [https://parts.igem.org/Part:BBa_K649301 BBa_K649301] containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +).<br />
 For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]