Difference between revisions of "Part:BBa K649301"
Takuya 1613 (Talk | contribs) |
Takuya 1613 (Talk | contribs) |
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Arginase is coded with Ptrc and RBS in this part.<br /> | Arginase is coded with Ptrc and RBS in this part.<br /> | ||
− | rocF is the same as BBa_K649300.<br /> | + | rocF is the same as [https://parts.igem.org/Part:BBa_K649300 BBa_K649300].<br /> |
Ptrc and RBS are from pTrc99A.<br /> | Ptrc and RBS are from pTrc99A.<br /> | ||
Revision as of 04:05, 27 October 2011
Ptrc-RBS-rocF
Arginase is coded with Ptrc and RBS in this part.
rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.
You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.
Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +). This part produced urea more efficiently in strain JD24293 (argR -).
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]