Difference between revisions of "Part:BBa K525405:Design"

 
(→‎Source)
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K525405 short</partinfo>
 
<partinfo>BBa_K525405 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Fusion Protein of BBa_K525403 and BBa_J18931
 
  
 +
* <partinfo>K525403</partinfo> fused to <partinfo>J18931</partinfo>
 +
** <partinfo>K525403</partinfo> synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
 +
 +
* NgoMIV restriction site downstream the start codon to easily fuse an N-terminal domain to the coding sequence
 +
 +
* AgeI restriction site upstream of stop codon to easily fuse a C-terminal domain to the coding sequence
  
  
 
===Source===
 
===Source===
  
−
Fusion Protein of S-Layer sbpA and mCitrine  
+
* <partinfo>K525403</partinfo> fused to <partinfo>J18931</partinfo>
 +
** <partinfo>K525403</partinfo> synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
 +
 
 +
* Promoter: fusion promoter between T7 promoter and lac-operator
 +
** T7 promoter from T7 phage
 +
** lac-operator from ''E. coli''
 +
 
 +
* <partinfo>K525401</partinfo> S-layer gene ''sbpA'' synthesized, originated in ''Lysinibacillus sphaericus'' CCM 2177
 +
 
 +
* <partinfo>J18931</partinfo> yellow fluorescent protein (YFP) mCitrine
 +
** from parts.igem
 +
** was synthesized before it was sent in
  
 
===References===
 
===References===
 +
Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of ''Lysinibacillus sphaericus'' CCM 2177 for the Generation of Functionalized Nanoarrays, ''Bioconjugate Chem'' [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903].

Latest revision as of 18:14, 26 October 2011

Fusion Protein of S-Layer SbpA and mCitrine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 221
    Illegal XhoI site found at 1996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 3913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 493
    Illegal BsaI.rc site found at 622


Design Notes

  • NgoMIV restriction site downstream the start codon to easily fuse an N-terminal domain to the coding sequence
  • AgeI restriction site upstream of stop codon to easily fuse a C-terminal domain to the coding sequence


Source

  • Promoter: fusion promoter between T7 promoter and lac-operator
    • T7 promoter from T7 phage
    • lac-operator from E. coli
  • BBa_K525401 S-layer gene sbpA synthesized, originated in Lysinibacillus sphaericus CCM 2177
  • BBa_J18931 yellow fluorescent protein (YFP) mCitrine
    • from parts.igem
    • was synthesized before it was sent in

References

Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of Lysinibacillus sphaericus CCM 2177 for the Generation of Functionalized Nanoarrays, Bioconjugate Chem [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903].