Difference between revisions of "Part:BBa K606040:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | ||
| + | This part have been synthetize de novo, by two 80bp primers matching on about 20bp, and completed using phusion polymerase, and then cloned in the pSB1C3 standard plasmid. | ||
===Source=== | ===Source=== | ||
| − | + | The sequence of this part vas inpire by the part BBa_K143015 from Cambridge, but without additional and non necessary sequences before and after the LacO. We created this part because the cambridge part was not available on the registry. | |
===References=== | ===References=== | ||
Latest revision as of 08:51, 26 October 2011
Promoter Hyperspank B.subtilis & E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part have been synthetize de novo, by two 80bp primers matching on about 20bp, and completed using phusion polymerase, and then cloned in the pSB1C3 standard plasmid.
Source
The sequence of this part vas inpire by the part BBa_K143015 from Cambridge, but without additional and non necessary sequences before and after the LacO. We created this part because the cambridge part was not available on the registry.