Difference between revisions of "Part:BBa K606010"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K606010 short</partinfo> | <partinfo>BBa_K606010 short</partinfo> | ||
| − | This part is | + | This part is designed to produce an expression of LacI in the B. Subtilis cell, in order to control the expression of the promoter pHyperSpank [https://parts.igem.org/Part:BBa_K606040 K1606040] level of expression. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | LacI gene is not expressed enough in B. Subtilis to be sufficient to regulate strongly the pHyperSpank promoter. This is a sub-part of the IPTG inducible expression cassette K606011 for Bacillus Subtilis, which contains the complete system | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K606010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K606010 SequenceAndFeatures</partinfo> | ||
Latest revision as of 22:17, 25 October 2011
LacI strong expression casette for B. Subtilis
This part is designed to produce an expression of LacI in the B. Subtilis cell, in order to control the expression of the promoter pHyperSpank K1606040 level of expression.
Usage and Biology
LacI gene is not expressed enough in B. Subtilis to be sufficient to regulate strongly the pHyperSpank promoter. This is a sub-part of the IPTG inducible expression cassette K606011 for Bacillus Subtilis, which contains the complete system
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]